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Molecular Biology Insights Oligo v7.54 ¤Þª«³]­p³nÅé ­^¤å¯}¸Ñª©




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OLIGO Primer Analysis Software is the essential tool for designing and        
analyzing sequencing and PCR primers, synthetic genes, and various kinds of   
probes including siRNA and molecular beacons.                                 
Based on the most up-to date nearest neighbor thermodynamic data, Oligo's     
search algorithms find optimal primers for PCR, including TaqMan, highly      
multiplexed, consensus or degenerate primers.  Multiple file batch            
processing is possible. It is also an invaluable tool for site directed       
mutagenesis.                                                                  
For each primer or primer pair, Oligo's various analysis windows show a       
multitude of useful data, such as DNA and RNA secondary structure, dimer      
formation, false priming and homology, internal stability, composition and    
physical properties.  With Oligo you can analyze open reading frames down to  
predicted molecular weight and pKa of proteins, and search for restriction    
enzyme sites, not only in DNA but also in reverse-translated proteins.        
The first version of the software appeared on the market in 1989. It went     
through several modifications, and the last one, the change from version 6    
to 7, was the most comprehensive. Oligo 7 can automatically select multiplex  
primers, process sequence files in batch modes, automatically design PCR      
primers to cover multiple DNA regions in just one search and automatically    
find primer/probe sets for real time PCR or finds nested primers sets. Oligo  
search protocols (scoring system) may be customized in detail, so you may     
optimize the results according to your specific needs.  



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